In the kiwi DNA isolation process, which step comes immediately after mixing washing up liquid and salt in water?

• A. Chop Kiwi (to break cell walls and allow the cytoplasm to leak out)
• B. Add kiwi pieces to mixture
• C. Denature enzymes by heating to 60°C for 15 minutes
• D. Blend mixture for 3 seconds

Answer: (B)

The key idea is that the detergent-salt solution is prepared to break open the kiwi cells and release the DNA into the liquid. Once that solution is ready, you need to introduce the kiwi tissue so the detergent can act on the cell membranes and nuclear envelopes. Adding the kiwi pieces to the mixture allows the cytoplasm and DNA to enter the solution.

Chopping the kiwi would occur earlier to increase breakage, heating to 60°C isn’t part of this typical extraction and could harm DNA, and blending is a preparatory step that isn’t the immediate action after the detergent solution is made.